HPLC法测定虫草发酵粉中黄曲霉毒素残留量

HPLC residual determination of afatoxins in a fermented cordyceps powder

目的:建立虫草发酵粉中黄曲霉毒素B1、B2、G1、G2的残留量测定方法。方法:样品经有机溶剂提取、免疫亲和柱净化后,利用高效液相色谱-光化学衍生-荧光检测器进行分析测定。结果:黄曲霉毒素G2、B2在1.5～150 pg范围内线性关系良好,黄曲霉毒素G1、B1在5～500 pg范围内线性关系良好,r>0.9998。回收率在80%～93%之间。结论:该方法快速简便,准确,可作为虫草发酵粉黄曲霉毒素的残留量测定方法,亦可作为原料药的黄曲霉毒素筛查方法,以达到控制产品质量的目的。

Objective：To estabilsh a rapid and accurate method for the residual determination of aflatoxins B1,B2,G1,G2 in a fermented cordyceps powder.Methods：After extracted by organic solvent,purified by immunoaffinity column,aflatoxins were analysed by HPLC with fluorescence detection after photochemistry derivation.Results：Aflatoxin G2,B2 showed a good linear relationship at a range of 1.5-150 pg,and aflatoxins G1,B1 at a range of 5-500 pg,r0.9998.The recovery was between 80%-93%.Conclusion：The method is simple,accurate and can be used to determine aflatoxins B1,B2,G1,G2 in a fermented cordyceps powder.It also can be used as raw material drug screening methods of aflatoxins in order to control product quality.