高效液相色谱法测定小鼠血浆头孢匹胺钠的含量

HPLC determination of cefpiramide sodium in rat plasma

目的:建立小鼠血浆中头孢匹胺钠HPLC检测方法,为临床上头孢匹胺钠血药浓度监测提供试验依据。方法:样品处理采用25%高氯酸(v/v)沉淀蛋白质。PLATISILTMC18(250 mm×4.6 mm,5μm)色谱柱,流动相为甲醇-三乙胺醋酸(三乙胺14 mL,冰醋酸5.7 mL,加蒸馏水定容至100 mL)-水(10∶0.2∶89.8),1 mol.L-1醋酸调节pH5.38,流速为0.8 mL.min-1;检测波长为254 nm。结果:所建立方法在0.2～250.0μg.mL-1范围内线性良好,方法平均回收率为96.1%,平均提取回收率为77.7%;日内变异RSD小于5%。小鼠尾静脉给药150 mg.kg-1后1 h血浆头孢匹胺钠浓度为(196.1±11.9)μg.mL-1,24 h血药浓度降至(0.5±1.6)μg.mL-1,采用DAS2.0程序求得药物动力学参数t1/2为3.41 h,MRT为3.24 h。结论:该法准确、灵敏、快速,内源性成分无干扰,适用于血浆中头孢匹胺钠的分析测定。

Objective：To develop an HPLC method for determination of Cefpiramide Sodium in rat plasma to provide evidence for the monitoring concentration of patients in plasma.Methods：The rat plasma samples were deproteimized with 25%（v / v） perchlorld acid.A PLATISILTM C18 column（250 mm ×4.6 mm,5 μm） with the mobile phase consisted of methanol-triethylamine acetic acid-water（10∶0.2∶89.8,v/v）,adjusted pH to 5.38 by 1mol·L-1 acetic acid was used to separate cefpiramide sodium in biological samples,and the detection occurred at 254 nm.Results：A good linearity was obtained in the concentration range of 0.2-250 μg·mL-1,the average recovery was 77.7%,the intra-day precision（RSD） was less than 5%.The concentration of cefpiramide sodium in rat plasma was（196.1±11.9） μg·mL-1 at 1.0 h after iv.150 mg·kg-1 cefpiramide sodium,and decreased to（0.5±1.6）μg·mL-1 at 24 h,The Pharmacokinetic parameters t1/2 was 3.41 h,MRT was 3.24 h.Conclusion：The described method was proved to be sensitive,rapid and accurate,can be applied in identification and determination of cefpiramide sodium in rat plasma.