酶法水解大豆膨化料提取多肽的工艺

Enzymatic Extraction of Peptides from Extruded Soybean

采用挤压膨化预处理水酶法提取大豆油的同时,也有较高的多肽得率。利用水酶法应用于大豆多肽的提取,并应用响应面优化方法得出大豆挤压膨化后水酶法提取多肽的最佳工艺为加酶量1.6%、酶解温度60℃、酶解时间3h、料水比1:5、酶解pH9.6。经过验证与对比实验可知,在最优酶解工艺条件下大豆多肽得率可达到41.36%左右,比相同酶解条件下未经挤压膨化预处理大豆多肽得率有显著提高。

Soybean extrusion before the aqueous enzymatic extraction of soybean oil can result in higher polypeptide yield. In this study, response surface methodology was employed to optimize process parameters for the aqueous enzymatic extraction of oil from extruded soybean in order to maximize soybean polypeptide yield. The optimal extraction conditions were alcalase amount of 1.6 %, temperature of 60 ~C, hydrolysis time of 3 h, material-to-liquid ratio of 1：5, and pH of 9.6. Under the optimal extraction conditions, the yield of soybean peptides was up to 41.36%, which revealed a significantly increase when compared with that obtained under the same extraction conditions without extrusion treatment.