补骨脂汤对血管性痴呆大鼠海马内BDNF/TrkB、GAP-43蛋白的影响

Influence of Buguzhi Decoction on BDNF/TrkB and GAP-43 in the Hippocampus of Vascular Dementia Rats

目的探讨补骨脂汤改善血管痴呆大鼠学习记忆的作用靶点。方法将60只SD大鼠随机分成假手术组、模型组、银杏叶片组、补骨脂汤低剂量组(补低组)、补骨脂汤高剂量组(补高组),每组12只,制备血管性痴呆大鼠模型,手术后第3d开始给药,采用二维电泳分离海马内总蛋白,用ImageMaster软件分析蛋白差异点,用蛋白印迹法、免疫组化分析差异蛋白的表达量。结果补高组、补低组总蛋白二维电泳的结果与模型组比较,发现差异蛋白点19个,其中与空间学习记忆相关的蛋白脑源性神经营养因子(BDNF)、生长相关蛋白-43(GAP-43)灰度比值及TrkB阳性细胞数补高量组明显高于模型组(P<0.05或P<0.01)。结论补骨脂汤可能通过提高BDNF/TrkB、GAP-43基因的表达水平,改善和治疗血管性痴呆者学习记忆能力。

Objective To explore the acting targets of Buguzhi Decoction （Decoction of psoralea fruit） in improving the learning memory of vascular dementia （VD）. Methods Sixty SD rats were divided into sham operation group, model group, gingko leaf tablet group, Buguzhi Decoction low dosage group, and Buguzhi Decoction high dosage group, 12 rats in each group. Medication was started since the third day after modeling. Two-dimensional electrophoresis was used to separate the total protein in the hippocampus of rats. Image Master software was adopted to analyze the differential protein spots, and protein molecularly imprinted method and immunohistochemical analysis were applied to detect the expression quantity of differential protein. Results The result of two-dimensional electrophoresis experiment shows that, there were 19 differential protein spots found in the total protein of the two Buguzhi Decoction groups, of them, the gray ratio between brain-derived neurotrophic factor （BDNF） and growth associated protein-43 （GAP-43） relating to space learning memory and Trk positive cell count were significantly higher than those of the model group （P〈0.05 or P〈0.01）. Conclusion Buguzhi Decoction improves the learning memory of VD rats possibly through increasing the expression level of BDNF/TrkB and GAP-43.