内毒素血症细胞因子一氧化氮在重度失血性休克发展中的作用

Studies on role of endotoximia, cytokines and nitric oxide in the development of prolonged hemorrhagic shock

目的 探讨内毒素血症、细胞因子、ＮＯ在重度失血性休克发展过程中的作用和机制。方法 选用大白兔２６只，分为失血性休克组（１４ 只），对照组（１２ 只），休克组观察休克前后血浆内毒素、ＴＮＦα、ＩＬ－ ６、ＩＬ－８、ＮＯ的动态变化，对照组观察手术前后上述指标的变化，两组动物均观察２４ 小时、４８ 小时存活率。结果 大白兔发生失血性休克后血浆内毒素、ＴＮＦα、ＩＬ－６、ＩＬ－８ 、ＮＯ的水平与休克前及对照组比较有明显升高，死亡动物中血浆上述物质水平显著高于存活动物。结论 内毒素血症、细胞因子、ＮＯ等发挥重要的协同作用，促使失血性休克向不可逆方向进展。

Objective To study the role of the mechanism of endotoxin, cytokines, NO in the development of prolonged hemorrhagic shock. Methods 26 rabbits were randomly divided into hemorrhagic shock group(14 rabbits), control group(12 rabbits). After establishing hemorrhagic shock model of rabbits, plasma levels of endotoxin, TNFα, IL-6, IL-8 and NO were detected at preshock and postshock. The survival rates of 24 h and 48 h were observed. Results The plasma levels of endotoxin, TNFα, IL-6, IL-8 and NO were markedly higher after shock than those of before shock and control group, and maintained at high level. The plasma levels of endotoxin, cytokines and NO in nonsurvivors were higher than those of surviving animals. Conclusions The endotoxin, cytokines and NO play an important role in the development of prolonged hemorrhagic shock. [