蒸汽爆破尾叶桉木材纤维素酶水解液发酵单细胞蛋白

Cell protein production by fermentation of cellulase hydrolysate from steam exploded Eucalyptus urophylla wood

用高效液相色谱对蒸汽爆破尾叶桉木材的纤维素酶水解液进行了还原糖和乙酸含量分析,通过酿酒酵母和假丝酵母混合接种,对水解液进行了摇瓶发酵单细胞蛋白的工艺研究,确定了发酵的最佳工艺条件。结果表明,水解液中主要含有葡萄糖和木糖,用双菌种混合发酵单细胞蛋白的最佳发酵条件为:酿酒酵母(Sacchromyces cerevisive):假丝酵母(Candida utilis2.587)为1:3,发酵温度31℃,初始pH4.5,初始还原糖浓度17～18g/L,发酵时间27h。

Reducing sugar and acetic acid in cellulase hydrolysate from steam exploded Eucalyptus urophylla wood were analyzed using high performance liquid chromatography,then single cell protein production in flask was studied with hydrolysate by two strains fermentation of Sacchromyces cerevisive and Candida utilis 2.587,and the optimum fermentation conditions were determined.The results showed that glucose and xylose were the major components in hydrolysate,the optimum fermentation conditions were：1：3 of the proportion of Sacchromyces cerevisive/Candida utilis 2.587,31 ℃ of fermentation temperature,initial pH 4.5 and 27 h of fermentation process.