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河南地区猪戊型肝炎病毒的检测及基因序列分析 被引量：3

Detection and sequence analysis of the swine HEV in Henan
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摘要 对从河南省郑州、洛阳、新乡、焦作、周口等5地市采集的133份血清样品进行戊型肝炎病毒(Hepatitis EVirus,HEV)IgG抗体分析;通过反转录聚合酶链式反应的方法(RT-PCR)对从郑州、中牟、新郑、济源、濮阳等5个地市采集的200份粪便样品进行HEV RNA检测.检测结果表明,79.70%的血清为HEV抗体阳性;7.5%的粪便样品为HEV RNA阳性.通过序列分析证明,3个测序的HEV河南分离株均为基因Ⅳ型.为了进一步研究河南HEV的特点,克隆得到筛选的地理株JY40病毒株基因组3’端826 bp的DNA片段,序列分析结果显示该片段与湖北株相应序列的同源性最高. 133 swine serum samples collected from Zhengzhou,Luoyang,Xinxiang,Jiaozuo and Zhoukou were tested by ELISA for the presence of IgG antibodies against HEV.200 pig fecal samples from Zhengzhou,Xinzheng,Zhongmou,Jiyuan and Puyang were tested for the presence of HEV RNA by reverse transcription nested polymerase chain reaction（RT-PCR）.Results showed 79.70% of the serums were positive for antibody against HEV,and 7.5% of the fecal samples were positive for HEV RNA.Nucleotide sequence analysis showed that three HEV strains detected in Henan belong to genotype Ⅳ.To get the characteristics of Henan HEV,we cloned 826 bp of 3＇ sequence from JY40 genome,and phylogeny analysis showed it had a high identity with Hubei strain.
作者 张小霞 许锋 梁振普 乔冠华
机构地区 河南农业大学生命科学学院
出处 《河南农业大学学报》 CAS CSCD 北大核心 2011年第3期302-306,共5页 Journal of Henan Agricultural University
基金 河南省重大公益性科研项目(81100912300)
关键词 猪 戊型肝炎病毒 检测 基因序列分析 swine HEV detection sequence analysis
分类号 S852.65 [农业科学—基础兽医学]
  • 相关文献

参考文献15

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二级参考文献20

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共引文献33

同被引文献26

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引证文献3

二级引证文献5

河南农业大学学报
2011年 第3期

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