龙眼LEAFY同源基因启动子的克隆与序列分析

Cloning and sequence analysis of LEAFY gene promoter from longan(Dimocarpus longan)

为了解龙眼LEAFY同源基因(LLFY)的表达调控规律,应用染色体步移方法克隆了809 bp的LLFY启动子序列。用在线软件PlantCARE分析表明,该序列除含有启动子基本元件,如TATA-BOX、CAAT-BOX外,还含有参与生理周期调控、干旱诱导MYB结合位点、ABA响应元件、光响应元件等一些其他的调控序列,推测LLFY的表达受生理周期、干旱、光照等的调控。用FootPrinter在线工具对龙眼等6个物种的LEAFY同源基因启动子进行比较,发现不同植物的启动子具有保守性,也存在差异,表明LEAFY同源基因功能上的分化,LLFY基因具有多种功能。

In order to study the expression and regulation rule of LEAFY homolog in longan（LLFY）,a 809 bp fragment of LLFY promoter was cloned by chromosome walking method.Promoter sequence analyzed by PlantCARE and PLACE showed that this promoter fragment had TATA-BOX,CAAT-BOX and some other regulatory elements,such as cis-acting element involved in circadian control,MYB binding site involved in drought-inducibility,cis-acting regulatory element involved in the abscisic acid responsiveness and light responsive element.It was supposed that the expression of LLFY was regulated by circadian,drought,light et al.Compared the promoter of LLFY with other 5 plants by FootPrinter analysis,these promoters had conserved domains while the distribution of transcription factor-binding site had difference.It implied the diversity of gene function of LEAFY genes,and LLFY may had different functions.