轮状病毒肠道内外感染患儿细胞免疫功能检测与分析

Analysis of cellular immunity in children with intestinal and extraintestinal rotavirus infection

目的探讨轮状病毒肠炎患儿肠道内、外感染时细胞免疫功能的变化。方法对2009年10月～2010年3月40例因轮状病毒肠道内、外感染住院的患儿,采用流式细胞仪检测外周血淋巴细胞亚群,并与20例正常婴幼儿进行比较。结果轮状病毒肠炎并发肠道外感染组患儿外周血CD4+、CD4+CD25+T淋巴细胞及CD4+/CD8+比值均增高,而NK细胞下降;轮状病毒肠炎肠道内感染组患儿外周血仅NK细胞下降。轮状病毒肠炎并发肠道外感染组患儿CD3+、CD4+、CD4+CD25+T淋巴细胞、CD19+B淋巴细胞比肠道内感染组组患儿高。结论轮状病毒肠炎并发肠道外感染组患儿细胞免疫功能明显紊乱,而肠道内感染组未见明显异常。免疫功能紊乱可能与轮状病毒肠道外播散存在一定关系。

Objective To investigate the change of cellular immunity in children with rotavirus enteritis with intestinal and extraintestinal manifestions.Methods The patients with rotavirus enteritis were enrolled between Oct.2009 and March 2010 for identification T lymphocyte subsets,the total B lymphocytes and the NK cells using flow cytometry.Results CD4＋、CD4＋CD25＋ T lymphocytes and the ratio of CD4＋/CD8＋ increased,and the NK cells decreased in children with rotavirus enteritis and extraintestinal manifestions as compared with the controls.In group of rotavirus enteritis and intestinal manifestions,only the NK cells decreased.Compared to the children with rotavirus enteritis and intestinal manifestions,CD3＋,CD4＋,CD4＋CD25＋ T lymphocytes CD19＋ B lymphocytes were higher in children with rotavirus enteritis and extraintestinal manifestions.Conclusion Cellular immunity in children with rotavirus enteritis and extraintestinal manifestions were obvious disturbance which might be relative to the extraintestinal spreading of rotavirus.