甘露醇灌胃对大鼠局灶性脑缺血再灌注损伤时的神经保护作用

Protective effect of mannitol gavage against cerebral ischemia-reperfusion injury in rats

目的:研究甘露醇灌胃给药对大鼠局灶性脑缺血再灌注损伤时的神经保护作用及机制。方法:采用大鼠大脑中动脉阻断模型,灌胃给予甘露醇进行干预。通过观察TUNEL染色细胞数量,测定细胞凋亡蛋白酶-3(caspase-3)、超氧化物歧化酶(SOD)活性,丙二醛(MDA)、8-羟基脱氧鸟嘌呤(8-OH-dG)和血中氢气含量等指标,观察甘露醇对大鼠脑缺血再灌注后脑神经的保护作用。结果:灌胃给予甘露醇后凋亡细胞明显减少,MDA和8-OH-dG含量、caspase-3及SOD活性明显降低,血中氢气的含量升高。结论:灌胃给予甘露醇可明显减少大鼠局灶性脑缺血再灌注损伤,可能通过肠道细菌产生氢气而发挥重要的脑保护作用。

Objective：To observe the neuroprotective effect of mannitol gavage against cerebral ischemia-reperfusion injury in rats.Methods： The neuroprotective effect of mannitol was verified by TUNEL staining.The TUNEL-positive cells were counted.The activities of caspase-3 and superoxide dismutase（SOD） were detected.The contents of malondialdehyde（MDA）,8-hydroxydeoxyguanine（8-OH-dG） and blood hydrogen in an established focal cerebral ischemia model were determined.Results： After mannitol gavage,the count of TUNEL-positive cells,the contents of MDA and 8-OH-dG were decreased.The activities of caspase-3 and SOD were reduced.The content of hydrogen in arterial blood was increased.Conclusion： Mannitol gavage can play an important role in brain protection.The role may be associated with the production hydrogen gas by the fermentation of gut bacteria.