用RP-HPLC法同时测定珍珠维E乳膏中维生素B_6和维生素E的含量

Simultaneous determination of vitamin B_6 and vitamin E in Pearl vitamin E cream by RP-HPLC method

目的：建立同时测定珍珠维E乳膏中维生素B6和维生素E的RP-HPLC法。方法：色谱柱为Kromasil-C18柱（150 mm×4.6 mm,5μm）,流动相为甲醇-0.2%磷酸溶液,梯度洗脱,检测波长285 nm,流速1.0 ml/min。样品在90%乙醇中用超声破碎仪破碎10 min,然后50℃水浴加热5 min处理。结果：维生素B6和维生素E的线性范围分别为1~50μg/ml（r=0.999 9,n=5）和1~50μg/ml（r=0.999 8,n=5）;平均回收率分别为（95.32±0.27）%（n=9）和（96.91±0.85）%（n=9）。3批样品中维生素B6和维生素E的含量分别为标示量的（94.91±0.77）%和（96.87±0.85）%。结论：该方法适用于珍珠维E乳膏中维生素B6和维生素E的含量测定。

Objective：To establish a RP-HPLC method for simultaneous determination of vitamin B6 and vitamin E in Pearl vitamin E cream.Methods： The determination was performed on a Kromasil-C18 analytical column（150 mm×4.6 mm,5 μm） with the mobile phase consisting of methanol and 0.2% phosphoric acid solution.The sample was eluted by gradient mode.The detection wavelength was 285 nm and the flow rate was 1.0 ml/min.The sample was extracted by ultrasonic grinder for 10 min in 90% ethanol solution,then heated in water bath at 50 ℃ for 5 min.Results： The standard curves were linear at the ranges of 1-50 μg/ml for both vitamin B6（r=0.999 9,n=5） and vitamin E（r=0.999 8,n=5）.The average recovery rates of vitamin B6 and vitamin E were（95.32±0.27）%（n=9） and（96.91±0.85）%（n=9）.The contents of vitamin B6 and vitamin E in three batches of samples were（94.91±0.77）% and（96.87±0.85）% respectively.Conclusion： The method can be used for simultaneous determination of vitamin B6 and vitamin E in Pearl vitamin E cream.