RP-HPLC法测定小儿清肺化痰口服液中槲皮素-3-O-β-D-葡萄糖-7-O-β-D-龙胆双糖苷的含量

Content Determination of Quercetin-3-O-β-D-glucose-7-O-β-D-gentiobioside in Xiaoer Qingfei Huatan Oral Solution by RP-HPLC

目的:建立测定小儿清肺化痰口服液中槲皮素-3-O-β-D-葡萄糖-7-O-β-D-龙胆双糖苷含量的方法。方法:采用反相高效液相色谱法。色谱柱为Diamonsil-C18柱,流动相为乙腈-0.1%醋酸水溶液(9∶91),流速为0.8mL·min-1,检测波长为260nm,进样量为10μL,柱温为25℃。结果:槲皮素-3-O-β-D-葡萄糖-7-O-β-D-龙胆双糖苷检测浓度在5～60μg·mL-1范围内与峰面积积分值具有良好的线性关系(r=0.9999);槲皮素-3-O-β-D-葡萄糖-7-O-β-D-龙胆双糖苷平均回收率为104.2%,方法精密度RSD=0.65%(n=6)。结论:该法分离效果好、灵敏、准确,可用于小儿清肺化痰口服液的质量控制。

OBJECTIVE ： To establish the method for the content determination of quercetin-3-O-β-D-glucose-7-O-β-D-gentiobi- oside in Xiaoer qingfei huatan oral solution. METHODS： RP-HPLC method was established. The chromatographic column was Di- amonsil-C18 column. The mobile phase was acetonitrile-0.1% acetic acid（9：91）at flow rate of 0.8 mL.min ＇. The detection wave- length was set at 260 nm and column temperature was 25 ℃. The injection volume was 10 μL. RESULTS： The linear range of quer- cetin-3-O-β-D-glucose-7-O-β-D-gentiobioside was 5~60 μg·mL ^-1（r=0.999 9） with an average recovery of 104.2% （RSD= 0.65%, n=6）. CONCLUSION： The method is well-separated, sensitive and accurate for the quality control of Xiaoer qingfei hua- tan oral solution.