宁夏肉牛杂交改良群体生长激素受体基因的多态性研究

Study on Polymorphisms of GHR Gene in Improved Hybrid Beef Cattle Groups in Ningxia Province

试验通过对宁夏六盘山区肉牛杂交改良群体生长激素受体(growth hormone receptor,GHR)基因的多态性研究,为宁夏肉牛杂交改良选育提供理论基础。采用PCR-RFLP对该群体70个个体GHR基因多态性进行检测,结果扩增出338 bp的目的片段,PCR产物被限制性内切酶AluⅠ消化后表现出多态性,共检测到2种基因型:AA、AB,其中A为优势等位基因,AA基因型为优势基因型。

Polymorphism of the growth hormone receptor（GHR） in improved hybrid beef cattle group from Liupan Mountain area in Ningxia province was investigated to give the rationale for hybrid improvement.Polymerase chain reaction-restriction fragment length polymorphisms（PCR-RFLP） technology was carried out to examine polymorphisms of GHR gene of 70 individuals.PCR products of 338 bp digested by restriction enzyme Alu Ⅰ showed polymorphisms.There were two genotypes of AA and AB.The frequency of A allele was higher than B allele,and the frequency of AA genotype was higher than AB genotype.