咽拭子与鼻咽抽吸液标本检测呼吸道感染患儿hRSV-DNA的比较研究

A comparative study on throat swabs and nasopharyngeal aspirates collected from children with respiratory infection for detection of respiratory syncytial virus

目的探讨比较反转录PCR(RT-PCR)法检测呼吸道感染患儿咽拭子和鼻咽抽吸液标本人呼吸道合胞病毒(hRSV)DNA的敏感性。方法收集200例急性呼吸道感染患儿的呼吸道标本(同时收集咽拭子和鼻咽抽吸液),应用针对hRSV的M基因保守区域的PCR引物进行hRSV基因片段检测,对阳性的标本进行比较分析。结果 200例患儿的呼吸道标本中(咽拭子和鼻咽抽吸液),共检出70例hRSV阳性标本,阳性率为35.0%。其中在鼻咽抽吸液标本中检出69例hRSV阳性标本,阳性率为34.5%;咽拭子标本中,共检出67例hRSV阳性标本,阳性率为33.5%;66例在咽拭子及鼻咽抽吸液中均检出hRSV,阳性率为33.3%。结论应用RT-PCR法检测hRSV,在咽拭子与鼻咽抽吸液标本间,阳性率差异无统计学意义。

Objective The objective of this study was to calculate sensitivity values for the detection of respiratory syncytial virus of childhood by using combined throat swabs and nasopharyngeal aspirates. Methods Two hundred specimens（ throat swabs and nasopharyngeal aspirates ） were collected from the children with acute respiratory infection. RT - PCR was performed to detect RSV from these specimens, using primers designed for the M gene from this virus. The positive RT - PCR products were selected for comparison. Results Seventy specimens （ throat swabs and nasopharyngeal aspirates） were RSV positive from the 200 specimens（ 35.0% ）. Sixty nine nasopharyngeal aspirates specimens were RSV positive （34.5 % ）. 67 throat swabs specimens were RSV positive （33.5 % ）. 66 paired throat swabs/nasopharyngeal aspirates specimens were RSV positive（33.3% ）. Conclusion There were no difference between throat swabs and.