人乳头状瘤病毒感染与p16蛋白表达在宫颈鳞癌中的意义

Significance of HPV infection and p16 expression in cervical squamous cancer

目的探讨人乳头状瘤病毒(HPV)感染与p16蛋白表达在宫颈鳞癌中发生、发展中的意义。方法收集2008年12月-2010年12月,72例宫颈鳞癌标本、32例上皮肉瘤变标本和28例正常宫颈组织标本,分别采用原位杂交法和免疫组织化学法,检测标本中HPV 16/18和p16蛋白的表达情况。结果 HPV16/18在宫颈鳞癌中的阳性率为59.7%,明显高于正常宫颈上皮的阳性率(7.1%)和宫颈上皮肉瘤变的阳性率(12.5%),经统计学分析,差异有统计学意义(均P<0.05),p16蛋白在宫颈鳞癌中的阳性率为79.2%,明显高于正常宫颈上皮的阳性率(7.1%)和宫颈上皮肉瘤变的阳性率(34.4%),差异有统计学意义(P<0.05),72例宫颈鳞癌组织中HPV16/18的表达与FIGO分期密切相关(P=0.038),而与患者年龄、肿瘤大小、细胞分化程度及淋巴结转移无关;p16蛋白的表达与FIGO分期、肿瘤大小及细胞分化程度密切相关(P<0.05),而与患者年龄及淋巴结转移无关。结论 HPV16/18和p16蛋白在宫颈鳞癌中表达明显升高,并且与宫颈鳞癌的临床病理资料密切相关,说明其在宫颈鳞癌的发生、发展中发挥着重要作用。

OBJECTIVE To detect the expression of HPV16/18 and p16 in cervical squamous cancer, and discuss their significances in genesis and development of cervical squamous cancer. METHODS From Dec 2008 to Dec 2010, 72 cases of cervical squarnous cancer samples, 32 cases of cervical intraepithelial neoplasia samples and 28 cases of normal cervical tissues samples were collected in this study. The HPV16/18 and p16 were detected by situ hybridization method and immunohistochemistry method, respectively. RESULTS The positive rate of HPV16/18 in cervical squamous cancer was 59. 7%, which was significantly higher than that in normal cervical tissues （7.1%） and cervical intraepithelial neoplasia（12.5 %）. The positive rate of p16 in cervical squamous cancer was 79.2%, which was significantly higher than that in normal cervical tissues （7.1%） and cervical intraepithelial neoplasia（34. 4%）. Of 72 cases with cervical squamous cancer, the expression of HPV16/18 was significantly associated with FIGO stage（P = 0. 038）, but showed no significant relationship with age, tumor size, cell differential degree and lymphoid node metastasis （P〉 0. 05）; the expression of p16 was significantly associated with FIGO stage, tumor size and cell differential degree（P〈0.05） but showed no significant relationship with age and lymphoid node metastasis （P 〉0. 05）. CONCLUSION The expression of HPV16/18 and p16 in cervical squamous cancer are significantly increased, and associated with clinical data, suggesting that they play important roles in genesis and development of cervical squamous cancer.