156株弗氏柠檬酸杆菌感染的分布与耐药性分析

Distribution and drug resistance of 156 strains of citrobacter freundii

目的了解医院临床标本分离的弗氏柠檬酸杆菌(CFR)检出分布规律及耐药性特点,为临床医师合理抗感染治疗提供依据。方法回顾性分析2008年1月-2010年5月,住院患者送检标本中分离的156株CFR,计算其构成比、科室分离率,鉴定按《全国临床检验操作规程》进行;药物敏感性试验采用CLSI推荐的K-B法。结果分离的156株CFR中,呼吸内科检出占39.1%,神经内科占15.4%,综合ICU占12.2%;主要标本来源于痰液、脓液、尿液,分别占总数的60.3%、21.2%、7.1%;病原菌检出447株,检出CFR 156株,占34.9%;CFR对氨苄西林、头孢唑林的耐药性较强,耐药率分别为100.0%、91.0%,可引起多部位感染。结论应重视对多药耐药CFR的监控与控制,预防医院感染暴发流行。

OBJECTIVE To understand the distribution and drug resistance of citrobacter freundii, to provide a reasonable basis for clinicians to use anti-infective therapy. METHODS Totally 156 strains of Citrobacter freundii from patients from Oct 2008 to May 2010 were analyzed, the component ratio and the isolation rate from various department were calculated by referring to the National Clinical Laboratory Operation Rules, as well as the drug sensitivity testing was performed by K-B methods. RESULTS Of 156 strains of isolated citrobacter freundii, the isolates from department of respiratory medicine accounted for 39.1%, from department of neurology 15.4 %, from intensive care unit was 12.2%, the main clinical specimens were collected from the sputum, the liquor puris and the urine, accounting for 60. 3%, 21. 2% and 7. 1%, respectively. Of 447 strains of detected pathogenic bacteria, there were 156 strains of citrobacter freundii, accounting for 34.9%; drug resistance rates of CFR to ampicillin and cefazolin were 100. 0% and 91. 0%, which could cause multiple sites infection. CONCLUSION Hospital should attach importance to the monitoring and the controlling of multidrug-resistant citrobacter freundii and prevent the outbreak and epidemic of nosocomial infections.