伤口分泌物病原菌培养及药敏分析

Bacterial culture from wound secretion and drug susceptibility

目的了解引起骨科术后伤口感染的病原菌分布及耐药性,为临床抗感染治疗提供参考。方法分析2007年1月-2009年12月送检的224份骨科伤口分泌物标本。结果共送检标本224份,培养阳性124份,阳性率55.3%;病原菌共检出156株,其中革兰阳性球菌80株占51.3%,革兰阴性杆菌65株占41.7%,真菌11株占7.0%;检出前5位的病原菌分别为金黄色葡萄球菌54株,大肠埃希菌22株,肺炎克雷伯菌14株,铜绿假单胞菌12株,假丝酵母菌属11株。结论对骨科伤口感染提倡针对药敏试验结果实行用药个体化。

OBJECTIVE To investigate the distribution and resistance of bacteria isolated from wound secretion in Orthopedics surgery in our hospital. METHODS The wound secretions were collected and cultivated from Jan 2007 to Dec 2009, antibiotic sensitivity tests were performed by Kirby-Bauer method. RESULTS A total of 224 wound secretion samples were collected, and 124 samples were culture positive. 156 bacterial strains were isolated, Gram- negative bacilli and Gram-positive cocci accounted for 41.7% （65 strains） and 51.3% （80 strains）, respectively, fungus 7%（11 strains）,The most common pathogens were Staphylococcus aureus （54 strains）, Escherichi coli （22 strains）, Klebsiella pneumoniae （14 strains）, Pseudornonas aerugionsa （12 strains） and Candida spp. （11 strains）. CONCLUSION It is important for clinical treatment of wound infection based on the bacterial culture and antibioticsusceptibility.