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干扰素λ诱导人食管癌YES5和T.Tn细胞抗增殖机制的研究 被引量：2

The mechanism of anti-proliferative activity induced by IFNλ in human esophageal carcinoma YES5 and T.Tn cells
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摘要 目的探查干扰素λ诱导人食管癌YES5和T.Tn细胞抗增殖的机制。方法流式细胞术检测细胞周期,膜联蛋白Ⅴ(annexinⅤ)和PI双染检测凋亡,Western印迹检测凋亡蛋白的表达。结果干扰素λ诱导人食管癌YES5和T.Tn细胞的亚G1期(sub-G1)升高,干扰素λ处理细胞与膜联蛋白Ⅴ的结合增加,干扰素λ可诱导细胞内半胱天冬酶(caspase)3的剪切,caspase抑制剂可减少干扰素λ诱导的sub-G1期升高。结论干扰素λ可诱导人食管癌YES5和T.Tn细胞发生凋亡。 ObjectiveTo investigate the mechanism of growth inhibition of IFN-lambda（IFNλ） in human esophageal carcinoma YES5 and T.Tn cells.MethodsThe profiles of the cell cycle were analyzed with flow cytometer.Staining with annexin and PI was used for testing apoptosis.The intracellular protein was detected with Western blotting analysis.Results IFNλ enhanced the sub-G1 and annexin Ⅴ＋ population in YES5 and T.Tn cells.IFNλ cleaved the caspase3.Caspase inhibitor eliminated the increased sub-G1 population induced by IFNλ.ConclusionIFNλ induces apoptosis in human esophageal carcinoma YES5 and T.Tn cells.
作者 赵鑫 齐义新 赵丹娜 杨正梅 卜友泉 魏林 李全海 胡洁
机构地区 河北医科大学第一附属医院 河北医科大学第四附属医院普外科 河北医科大学基础医学院免疫学教研室 重庆医科大学分子医学与肿瘤研究中心
出处 《军事医学》 CAS CSCD 北大核心 2011年第6期451-453,476,共4页 Military Medical Sciences
基金 国家自然科学基金资助项目(81000913 81001097) 河北省国际科技合作计划项目(10396101D)
关键词 干扰素类 抗增殖 细胞凋亡 食管肿瘤 interferons anti-proliferation cell apoptosis esophageal neoplasms
分类号 R735.1 [医药卫生—肿瘤]
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参考文献1

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同被引文献53

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军事医学
2011年 第6期

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