利用气质联用方法研究功能性消化不良患者血浆代谢谱的变化

Metabolic changes in plasma of patients with functional dyspepsia using GC-MS

目的采用基于气质联用的代谢组学技术研究功能性消化不良(functional dyspepsia,FD)患者血浆中小分子代谢物的变化。方法采集FD女性患者血浆样本16例及正常女性14例,经气相色谱-飞行时间质谱(gaschromatography/time-of-flight mass spectrometry,GC/TOF-MS)联用检测后,应用SIMCA-P10.0软件对积分数据进行偏最小二乘法-辨别分析(partial least squares-discriminant analysis,PLS-DA)。结果 GC/TOF-MS共检测得到色谱峰122个,其中鉴定出代谢产物31个。统计分析结果显示,与正常人相比,FD女性患者血浆中尿素、甘氨酸、苯丙氨酸、缬氨酸、磷酸、亚油酸、胆固醇等代谢物含量发生了明显变化。结合代谢途径分析发现,FD患者体内的能量代谢升高,而且患者体内氨基酸、脂肪酸以及肠道菌群等代谢也受到影响。结论本文所采用的气质联用代谢组学方法能清晰地反映FD女性患者血浆中的代谢物变化,其所揭示的代谢途径变化使得该技术在发病机制的探讨上具有良好的应用前景。

ObjectiveTo investigate the characteristics of plasma metabolites in functional dyspepsia（FD）.Methods Gas chromatography/time-of-flight mass spectrometry（GC/TOF-MS）-based metabonomics was applied to compare the plasma metabolic difference between FD female patients（n=16） and normal female subjects（n=14）.The integral data were subject to partial least squares-discriminant analysis（PLS-DA） through SIMCA-P10.0 software.ResultsUsing GC/TOF-MS technique,a total of 122 chromatographic peaks were detected,35 of which were identified as endogenous metabolites.Multivariate statistical analysis showed significant changes in FD plasma metabolites,such as those of urea,glycine,phenylalanine,valine,linoleic acid,cholesterol compared to healthy controls.Analysis of metabolic pathway found that FD female patients had a higher level of energy metabolism than controls.Besides,metabolism of amino acids,fatty acids and intestinal flora were affected in FD female patients.ConclusionThe metabonomic approach can evaluate the level of plasma metabolites in FD patients.This new method may be of great benefit to the pathogenesis research of FD.