摘要
采用CTAB法对基物培养获得的香蒲拟发网菌(Stemonitopsis typhina(Wiggers))进行总DNA提取,并用引物对其12S rDNA片段进行扩增,扩增产物序列长度为376 bp,探讨了香蒲拟发网菌的分子系统学关系。
The CTAB method was used for total DNA extraction from Stemonitopsis typhina (Wiggers) obtained by means of the moist chamber culture. The rDNA of S. typhina was amplified by 12S primers and then sequenced. The length of the sequence is 376 bp. The phylogeny of S. typhina was discussed.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2011年第7期96-98,共3页
Journal of Northeast Forestry University
基金
国家自然科学基金项目(70670015&71070012)
关键词
香蒲拟发网菌
12S
RDNA
PCR
分子系统学
Sternonitopsis typhina
12S rDNA
Polymerase chain reaction
Molecular phylogeny