甜荞查尔酮合酶基因Chs的克隆及序列分析

Cloning and sequence analysis of chalcone synthase gene Chs from Fagopyrum esculentum

利用RT-PCR技术从甜荞中克隆得到查耳酮合酶（CHS）的cDNA开放阅读框（ORF）序列,命名为FeChs,NCBI登录号为GU172166.1。该序列长1 179 bp,编码392个氨基酸,与其它植物CHS基因的同源性为78%~92%,其推导的氨基酸序列含有CHS高度保守的活性位点及CHS的标签序列GFGPG。

The cDNA ORF sequence of chalcone synthase gene（FeChs）was amplified by RT-PCR from Fagopyrum esculentum.Its accession number is GU172166.1 on GenBank.The anlyasis indicated the cDNA sequence was 1 179 bp,encoding 392 amino acids and had a homlogy 78%-92% with other plants＇CHS genes.The deduced amino acids sequence of FeChs contained the highly conserved catalytic active sites,and the typical Tag sequence of CHS with GFGPG.