HSV-tk基因转导对涎腺腺样囊性癌细胞生物学特性的影响

Biological Characters of Adenoid Cystic Carcinoma Cells Transduced\;with HSV tk Gene

目的:检测单纯疱疹病毒胸苷激酶(HSV-tk)基因的转导对高转移性涎腺腺样囊性癌细胞(ACC-M)生物学行为的影响。方法:对基因转导细胞作生长曲线测定、电镜观察、细胞周期检测、细胞表面组织相容复合物(MHC)类和类分子表达变化的测定。结果:HSV-tk基因转导组和亲本肿瘤细胞组无明显差别。

Objective: To study the effects of herpes simplex virus thymidine kinase(HSV tk) gene transduction on adenoid cystic carcinoma(ACC) cells. Methods: Gene transduced cells were detected with growth rate, scanning electronic microscope, transmission electronic microscope, cell cycle analysis and the expression of major histocompatibility complex(MHC) class Ⅰ or Ⅱ molecules. Results: The parameters detected in gene transduced cells and parental tumor cells were similar. Conclusion: The HSV tk gene transduction would not change the biological characters of tumor cells greatly.\;