儿童唾液中蛋白水解酶活性的分析测定

The Study of the Activities of Salivary Proteolytic Enzymes in Children

目的:分析测定儿童唾液中的蛋白水解酶活性,研究其与性别和龋敏感性的关系。方法:采用酶-底物反应法和考马斯亮蓝蛋白含量测定法,分别测定不同性别、不同龋敏感性儿童唾液中亮氨酸氨肽酶、甘氨酰脯氨酸二肽酶和胰酶样蛋白酶的活性及总蛋白含量。结果:1在儿童唾液中仅能测得甘氨酰脯氨酸二肽酶和胰酶样蛋白酶,且其活性明显低于牙菌班中的酶活性(P<0.05);2唾液中蛋白水解酶活性与儿童性别和龋齿数无关(P>0.05)。

Objective:The activities of proteolytic enzymes in saliva were assayed to determine whether they were related to caries sensitivity and gender in the children. Methods: Fluorometric and photometric methods were applied. Three enzymes included leucine amino peptidase, dipeptidyl peptidase Ⅳ and trypsin like proteinase. Results:①Only dipeptidyl peptidase Ⅳ and trypsin like proteinase were demonstrated in saliva, they were obviously lower than those in dental plaque (P<0.05); ②The activities of proteolytic enzymes of male and female children as well as caries active and caries free children showed no difference. Conclusion:Proteolytic enzymes play little role in caries formation of deciduous tooth.\;