摘要
目的：探讨三氧化二砷（Ａｓ２Ｏ３） 治疗慢性粒细胞性白血病（ＣＭＬ） 的机制。方法：以ＣＭＬ细胞系Ｋ５６２ 为模型，通过细胞增殖、活力检测、形态学观察、肿瘤集落形成的琼脂糖凝胶电泳等检验。结果：经≥２．５μｍｏｌ／ＬＡｓ２Ｏ３ 处理的Ｋ５６２ 细胞可出现增殖受抑和凋亡的形态学改变及ＤＮＡ片段化。结论：Ａｓ２Ｏ３ 能有效地诱导Ｋ５６２ 细胞凋亡。
To explore the mechanisms of arsenic trioxide(As 2O 3) in treatment of chronic myelocytic leukemia(CML), K 562 cell line was used as an in vitro model. The effect of As 2O 3 on K 562 cells was observed by cell proliferation, cell viability, cell morphology,tumor colony formation and agarose gel electrophoresis assay. The results showed that As 2O 3 at a concentration greats or equal 2.5μmol/L could inhibit the cell proliferation and induce the apoptotic morphology and DNA fragmentation of K 562 Cells. These results suggest that As 2O 3 can induce the apoptosis of K 562 cells efficiently.
出处
《苏州医学院学报》
1999年第11期1156-1158,共3页
Acta Academiae Medicinae Suzhou
基金
苏州医学院附属一院青年骨干基金
