鲨鱼软骨粉(SCP)合剂诱导的抗血吸虫性肝纤维化效应机制的研究

Studies on the Mechanism of Anti-Schistosome Hepatic Fibrosis Induced by SCP Combining Reagent

目的：了解ＳＣＰ合剂诱导的抗肝血吸虫性纤维化的效应机制。方法：用放射免疫分析法（ＲＩＡ） 和酶联免疫吸附试验（ＥＬＩＳＡ）检测血清透明质酸（ＨＡ） 和层粘连蛋白（ＬＮ） 水平。结果：血吸虫感染小鼠服用ＳＣＰ合剂３周（感染７ 周）后，ＳＣＰ合剂大剂量（９０ｍｇ／ｄ）组、小剂量（７０ｍｇ／ｄ）组和ＳＣＰ单剂组血清ＨＡ水平分别为０．５４±０．１６、０ ．９１ ±０．３０ 和１．７７±０．２７ｍｇ／Ｌ，均显著低于“４２５”单剂组和感染对照组的２．１４ ±１ ．２２ 和２ ．６５±０．７６ｍｇ／Ｌ（Ｐ＜ ０．０１） ；ＳＣＰ合剂大剂量组ＨＡ水平明显低于小剂量组和ＳＣＰ单剂组（Ｐ＜０．０１） ，而单用“４２５”制剂组ＨＡ 含量则与感染对照组之间无显著差异（Ｐ＞ ０．０５）。相应时期ＳＣＰ合剂大剂量组、小剂量组、ＳＣＰ单剂组和“４２５”单剂组血清ＬＮ水平（ＯＤ值）分别为０．７０±０．０４ 、０．８４ ±０．０５、１．０５±０．０５ 和０．８３ ±０ ．０４，均显著低于感染对照组的１ ．５９±０．１０（Ｐ＜ ０．０１），前４ 组血清ＬＮ水平之间均无显著差异（Ｐ＞０．０５）。结论：鲨鱼软骨粉合剂可降低血吸虫感染宿主血清ＨＡ和ＬＮ水平，阻止成纤维母细?

The levels of hyaluronic acid (HA) and laminin (LN) were detected by radioimmunoassay (RIA) and ELISA respectively probe the mechanism of anti-schistosome egg granuloma and anti-hepatic fibrosis induced by shark cartilage powder (SCP) combining reagent in murine schistosomiasis. Serum HA and LN levels were measured in BALB/c mice with schistosomiasis 3 weeks after orally administering SCP combining reagent (7 weeks after infection).The mean serum HA levels in the group with larger dosage of SCP combining reagent (group I) (0.54±0.16mg/L), smaller dosage of SCP combining reagent (group II) (0.91±0.30mg/L ) and single SCP (group III) (1.77±0.29mg/L) were significantly lower than that in the group of sinlge “425” (group IV,2.14±1.22mg/L) and in the infection controls (group V,2.65±0.76mg/L), and the serum HA level in group I was significantly lower than that in group II and IV (P< 0.01 ).But the serum HA level in group IV didn't differ from that in the control group V (P>0.05). The serum LN levels (OD value) in the above mentioned groups from I to V were 0.70±0.04, 0.84±0.05, 1.05±0.05, 0.84±0.04 and 1.58±0.10 respectively, and a significant difference in the mean serum LN levels in group I to IV was showed in comparison with the level in group V (P<0.01). But no significant difference was observed among the group from I to IV (P>0.05). The results suggest that the SCP combining reagent can decrease the levels of serum HA and LN in host infected with Schistosoma japonicum. The results indicate that the mechanisms of anti-hepatic fibrosis effects induced by SCP combining reagent is associated with inhibition of HA and LN secretion.