四逆汤抗犬急性心肌缺血的实验研究

Experimental research on the protective effect of SiNi decoction on acute ischemic myocardium

目的：观察四逆汤对急性心肌缺血犬心内膜心电图及心肌酶学脂质过氧化损伤等指标的影响。方法：应用结扎犬冠状动脉的方法造成急性心肌缺血模型，描记心电图，检测磷酸肌酸激酶（ＣＰＫ） 、磷酸肌酸激酶同功酶（ＣＰＫ－ＭＢ） 、谷丙转氨酶（ ＡＬＴ） 、乳酸脱氢酶（ＬＤＨ） 、超氧化物歧化酶（ＳＯＤ） 、丙二醛（ ＭＤＡ） 等指标。结果：四逆汤能显著降低急性心肌缺血犬心电图缺血范围和缺血程度，降低血ＣＰＫ、ＣＰＫ－ ＭＢ、ＡＬＴ、ＬＤＨ 等酶的活性，提高ＳＯＤ 活性，降低ＭＤＡ 含量。

AIM:To observe the effect of SiNi decoction on electrocardiography(ECG), enzymes and superoxide dismutase in acute ischemic canine myocardium. METHODS:The left anterior descending cornary artery was ligated to build a model of acute ischemic myocardium.The following items were examined:①ECG;②superoxide dismutase(SOD) and malondialdehyde(MDA);③alanine aminotransferase(ALT),lactate dehydrogenase(LDH),creatine phosphokinase(CPK) and CPK-MB.RESULTS:SiNi decoction reduced N-ST and ∑-ST of acute ischemic canine myocardium significantly.The activities of CPK,CPK-MB,ALT and LDH of SD groups are very much lower than those of control group.SiNi decoction also increased the SOD activity and reduced the concentration of MDA.CONCLUSION: SiNi decoction has protective effect on acute ischemic canine myocardium.