雷公藤对哮喘豚鼠外周血淋巴细胞IL-5、粒细胞-巨噬细胞集落刺激因子mRNA及CD4^+、CD8^+表达的影响

Effect of tripholide on peripheral blood lymphocytes IL-5, GM-CSF mRNA and CD4^+,CD8^+ expression in allergic guinea pigs

目的：探讨哮喘豚鼠白介素－ ５（ＩＬ－ ５） 、粒细胞－ 巨噬细胞集落刺激因子（ ＧＭ － ＣＳＦ） 及ＣＤ４ ＋ 和ＣＤ８ ＋ Ｔ 细胞在哮喘发病中的作用及雷公藤的干预作用。方法：实验分为哮喘组、雷公藤治疗组（ 处理组） 和对照组，每组各１０ 只豚鼠，采用原位杂交方法检测豚鼠外周血淋巴细胞的ＩＬ－ ５ 、ＧＭ－ ＣＳＦ ｍ ＲＮＡ 表达；应用免疫细胞化学方法检测外周血淋巴细胞ＣＤ４ ＋ 和ＣＤ８ ＋ 的表达。结果：哮喘组外周血淋巴细胞ＩＬ－ ５ 、ＧＭ－ ＣＳＦ－ ｍ ＲＮＡ 表达均明显高于处理组和对照组（ Ｐ＜ ０－０１） ，而处理组和对照组无显著差异。哮喘组外周血淋巴细胞ＣＤ４ ＋ 表达明显高于对照组和处理组（ Ｐ＜０－０１） ，ＣＤ８ ＋ 表达低于对照组和处理组（ Ｐ＜ ０－０５） 。结论：哮喘豚鼠外周血淋巴细胞ＩＬ－ ５ 、ＧＭ － ＣＳＦ ｍ ＲＮＡ 表达增高、ＣＤ４ ＋ 表达增高，ＣＤ８ ＋ 表达降低，而雷公藤甲素可降低外周血淋巴细胞ＩＬ－ ５ 、ＧＭ － ＣＳＦ ｍＲＮＡ 表达，并可提高ＣＤ８ ＋ 表达，降低ＣＤ４ ＋ 表达。

AIM:To explore the action of interleukin-5 (IL-5), granulocyte/macrophages colony-stimulating factor (GM-CSF), CD4 T cell and CD8 T cell on the allergic guinea-pigs. METHODS:The guinea-pigs were divided into three groups as following (1) Asthmatic group,(2) Tripholide-treated group, (3)Control group. Each has 10 guinea-pigs. The expression of IL-5 and GM-CSF mRNA in peripheral blood, the expression of peripheral blood CD4 + and CD8 + were determined with immuno chemistry techniques. RESULTS:The expression of IL-5 and GM-CSFmRNA of peripheral lymphocytes in asthmatic group markedly increased than that in tripholide-treated group and control group( P<0 01 ).There was no difference between the tripholide-treated group and the control group,but the expression of CD8 was much lower than that in the control group and the tripholide group. CONCLUSION:Tripholide may play a role in inhibiting airway inflammation in asthma.