重组人碱性成纤维细胞生长因子对人羊水细胞及其它细胞的影响

Effect of recombinant human basic fibroblast growth factor on human amniotic fluid cell and other cells

目的：探讨重组人碱性成纤维细胞生长因子（ｒｈｂＦＧＦ） 对人羊水细胞及其它细胞的影响。方法：在低血清培养基培养条件下进行人羊水细胞及Ｂａｌｂ／ｃ 小鼠巨噬细胞和３Ｔ３ 成纤维细胞，采用四甲基偶氮唑盐（ ＭＴＴ） 比色法和倒置显微镜观察ｒｈｂＦＧＦ 对人羊水细胞存活的影响。结果：人羊水细胞、Ｂａｌｂ／ｃ 小鼠巨噬细胞和３Ｔ３ 成纤维细胞在０－４ ％ 胎牛血清培养基培养条件下，不同浓度的ｒｈｂＦＧＦ 对羊水细胞的作用存在相关性。结论：ｒｈｂＦＧＦ 能延长羊水细胞、Ｂａｌｂ／ｃ小鼠巨噬细胞和３Ｔ３

AIM : To study the action of recombinant human basic fibroblast growth factor (rhbFGF) on human amniotic fluid cell and other cells. METHODS: The survival effect rhbFGF on amniotic fluid cell,Balb/c mouse macrophage and 3T3 fibroblast in a low-serum HamF10 were investigated by using MTT method and an contrast inverted microscopy. RESULTS: Following a series of different rhbFGF concentrations. They exerted their related action respectively on these cells in 0.4% fatal bovine serum HamF10. CONCLUSION: The survival of human amniotic fluid cell, Balb/c mouse macrophage and 3T3 fibroblast could be prolonged by rhbFGF.