硫化氢预处理延迟相对大鼠心肌蛋白质表达的影响

Proteomic Analysis of Hydrogen Sulfide Delayed Preconditioning on Rat Myocardium with TwoDimensional Polyacrylamide Gel Electrophoresis

【目的】研究硫化氢预处理对大鼠心肌蛋白质表达的影响。【方法】采用双向凝胶电泳技术对硫化氢预处理组（H组）大鼠心肌与对照组（C组）大鼠心肌蛋白质进行分离和比较分析。【结果】C组的蛋白质点数平均为929±14，H组的蛋白质点数平均为906±10。有15种蛋白质在硫化氢预处理后发生了改变，其中表达增强的有6个蛋白，表达降低的有9个蛋白。【结论】这些差异表达的蛋白质可能参与了硫化氢预处理对心肌的保护作用。

[Objective]To investigate the effect of hydrogen sulfide preconditioning on the protein expression of rat rnyocardium. [Methods] Two dimensional polyacrylamide gel electrophoresis（2-DE） was used to ex tract and comparatively analyze the proteins in rat myocardium of hydrogen sulfide preconditioning group （group H） and control group（group C）. [Results] The protein spots in group C and group H were 929±14 and 906±10, respectively. After hydrogen sulfide preconditioning, 15 proteins changed. of these 15 proteins, the expression of 6 proteins increased and that of 9 proteins decreased. [Conclusion] These differential proteins may be involved in the eardioproteetion of hydrogen sulfide preconditioninu.