木质纤维素降解菌系的筛选及其降解特性

Selection of composite microbial system decomposing lignocellulose and analysis of their decomposing characteristics

旨在为秸秆类木质纤维素沼气工业化奠定科学基础,以滤纸作为纤维素材料对不同发酵原料和发酵时期的沼气池菌系进行驯化和筛选,并研究其中温条件下对麦秸、玉米秸和滤纸的降解特性.采用减重法、分光光度法和蒸馏法分别测定复合菌系在发酵过程中的纤维素降解能力、复合菌系生物量和发酵液中挥发性有机酸.结果显示:发酵原料和发酵时期的菌源纤维素降解能力有较大差异,来自粪便池源的复合菌系在发酵前期纤维素降解力强,而秸秆池源的复合菌系在发酵后期纤维素降解力强.粪源产气稳定期的复合菌系F6的降解能力最强,对纤维素降解能力由高到低依次为滤纸、麦秸和玉米秸.

The aim of the paper is to provide the scientific basis for lignocellulose biogas industrialization.Biogas project plays an important role in energy and environment.Straw lignocellulose is regarded as material for biogas production.The study was conducted to select composite microbial system decomposing lignocellulose from biogas liquid at different fermentation time and raw material using filter paper as cellulose material,and further to understand their degradation characteristics to filter paper,wheat straw sand maize straw.Weight-loss method,spectrophotometry and distillation method were used to determine cellulose degradation rate,biomass of the composite strains and the content of volatile organic acids in fermentation broth during fermentation.The results showed that there were differences in cellulose degradation and physical and chemical characteristics of the composite strains from different fermentation time and raw materials.The composite strains from farce material had stronger degradation rate during fermentation early stage.The composite strains from crop straw material showed higher degradation rate during fermentation late stage.The composite strains F6 from the biogas liquid during biogas production stable stage had the strongest degradation of filter paper,followed by wheat and maize straw.