HPLC法测定生脉注射液中5-羟甲基糠醛的含量

Determination of 5-Hydroxymethyl-furfural in Shenmai Injection by HP LC

目的：建立生脉注射液中5-羟甲基糠醛的含量测定方法。方法：采用高效液相色谱法，色谱柱为Phenomenex Gemini C18（250mm×4．6mm，5μm），流动相为甲醇-水（5：95），检测波长为284nm，流速为1．0ml·ml^-1。结果：5-羟甲基糠醛的线性范围为1．981～118．860Wg（r=0．9998）；浓度范围内进样量与峰面积线性关系良好。平均回收率分别为101．28％（RSD=1．12％）（n=6）。结论：所建方法简单、准确，可用于生脉注射液中5-羟甲基糠醛的含量测定。

Objective： To establish a method for determination of 5 - Hydmxymethyl - furfural in Shenmai Injection. Method： The separation was performed on Phenomenex Gemini C18 （ 250mm × 4. 6mm, 5μm ） columm with detection wave length of 284 nm. The mobile phase consisted of methanol - water （5：95 ） with flow rate of 1.0 ml · ml^-1. Result： The linear ranges of 5 - Hydroxym- ethyl - furfural were at 1.981 - 118. 860 p,g （ r = 0. 9998 ）, the average recoveries （ n = 6 ） were 101.28 % （ RSD = 1.12% ） o Conclusion： This method is simple, accurate and suitable for the determination of 5 - Hydroxymethyl - furfural in Shenmai Injection.