分离纯化豌豆核DNA的有效方法

An Useful Method of Separation and Purication for Nuclear DNA of Pea

通过改进豌豆核ＤＮＡ的分离纯化方法，简化了分离纯化豌豆核ＤＮＡ的操作程序；纯化了的Ｇ２ 豌豆核ＤＮＡ 的紫外吸收值Ａ２６０／ Ａ２８０ ＝ １ ．９２ ，Ａｌａｓｋａ 豌豆核ＤＮＡ的紫外吸收Ａ２６０／ Ａ２８０ ＝ １ ．８８， 表明该方法提取的豌豆核ＤＮＡ是高纯度的ＤＮＡ，根据光吸收值测定产量收率，从每克豌豆幼苗中可获得约１３０ μｇ 核ＤＮＡ，这为豌豆核ＤＮＡ 的有关分子生物学研究提供了简便、经济、有效的分离纯化方法．

An improved method of separation and purification of nuclear DNA from pea tissue is described in this paper, which simplifies working processes. A 260 /A 280  of the purified nuclear DNAs of G 2 pea and Alaska pea are 1.92 and 1.88 respectively, these are typical value of absorption ultraviolet spectrum of nucleic acieds. It clearly showed that purified nuclear DNA are pure DNA. The output of nuclear DNA is about 130 micrograms per gram of seedling. This method is simple, economic and useful for further study on the nuclear DNA of pea.