高效液相色谱法测定人血浆中帕珠沙星浓度

High performance liquid chromatography (HPLC) to human plasma concentration of pearl sand pato

目的建立HPLC-紫外法检测人血浆中帕珠沙星浓度的方法。方法采用25%高氯酸溶液沉淀蛋白,分析柱为CNWSILC18（4·6mm×100mm）,流动相为0·02mol/L磷酸二氢钠溶液-乙腈-三乙胺（86∶13·5∶0·5）,pH=3·5,紫外测定波长248nm。结果帕珠沙星浓度在0·08~30·96mg/L呈线性关系,r=0·9998（n=6）;萃取回收率高于88%,方法回收率98%~101%,日间日内精密度均小于6%。在-40℃冰箱保存15d或冻融2次稳定性较好。结论 本方法简便灵敏,适合用来检测血浆中帕珠沙星浓度。

Objective To establish HPLC ultraviolet method to detect human plasma concentrations of pearl sand pato method.Methods Using 25% high chlorine acid solution protein deposition, analysis for CNWSILC18 （4.6 mm column 100mm）,mobile phase （mol/L for 0.02 sodium dihydrogen phosphate solution-acetonitrile-three procedures （86∶13.5∶0.5）,pH= 3.5,uv wavelength 248nm determination.Results Palmer bead uptake in 30.96 0.08-concentration of the linear mg/L,r=0.9998（n= 6）;Extraction recovery higher than 88%, method,daytime 101% recovery98-are less than 6% within precision.In-40 ℃ cross-matched 15d or freeze-thaw 2 times stability is good.Conclusion This method is suitable for sensitive,and Jane pato in plasma pearl sand detection concentration.