5种大量元素对青钱柳愈伤组织生长及黄酮类化合物积累的影响

Effects of Five Macronutrients on the Growth and Flavonoid Accumulation of Cyclocarya paliurus Callus

以MS为基本培养基,研究不同浓度的5种大量元素对青钱柳愈伤组织生长及黄酮类化合物积累的影响。结果表明:MS培养基中NH4NO3、KNO3浓度分别降至1/2C时,有利于愈伤组织生长和黄酮积累;当KH2PO4浓度为3/2C时,愈伤组织生长量与黄酮含量均达到最大值,分别为0.53 g/瓶和4.67%;MgSO4浓度在3/2C时,愈伤组织生长良好,而1/2C有利于黄酮积累;CaCl2在一定范围内不会影响愈伤组织生长,适当提高Ca2+浓度有利于黄酮积累。

The effects of five macronutrients in MS agar medium on the growth and flavonoid accumulation of Cyclocarya paliurus Callus were studied.The results showed that decreasing the concentrations of NH 4NO 3 and KNO 3 to 1/2C in MS medium favored the growth and flavonoids accumulation of callus.In contrast,3/2C KH 2PO 4 was the best concentration of medium,in which the dry weight of callus and flavonoids concentration were 0.52 g/bottle and 4.67%,respectively.The effect of MgSO 4 concentration showed that medium with 3/2C MgSO 4 was suitable for callus growth,while for flavonoid accumulation 1/2C MgSO 4 was better.CaCl 2 had no significant effect on callus growth.However,increasing Ca2＋ concentration improved flavonoids accumulation in the medium.