三种抗肿瘤中药有效成分对人脐静脉内皮细胞生长的影响

The impact of active ingredients of three anti-tumor Chinese medicines on the growths of human umbilical vascular endothelial cells

目的探讨蜂毒素、去甲斑蝥素、蟾毒灵3种抗肿瘤中药提取物的抗血管生成作用,并检测其量效关系,比较其抑制血管生成所需浓度与抑瘤浓度的关系。方法采用四甲基偶氮唑蓝(MTT)法测定3种药物在不同浓度、时间对人肝癌细胞ECV304生长的抑制作用,计算半数抑制浓度(IC50),观察量效、时效关系。结果 3种药物对ECV304细胞的生长有不同程度抑制作用(P<0.05或P<0.01),且呈浓度和时间的依赖性。蜂毒素在24、48和72h抑制ECV304细胞的IC50分别为7.64、4.25和2.64μg/ml,去甲斑蝥素为271.52、108.83和80.76μg/ml,蟾毒灵为1.104、0.355和0.0905μg/ml。结论蜂毒素、去甲斑蝥素、蟾毒灵有明确的抑制血管内皮细胞增殖、抗血管生成的作用。

Objective Melittin,norcantharidin and bufalin are three extracts from anti-tumor Chinese medicine that involve very great cytotoxic effects,whereas there is still no further research as to whether they are associated with anti-angiogensis.This study aims to investigate the presence of anti-angiogensis activities of these three extracts and detect their dose-effect relationships.Moreover,the correlations of the required concentration to induce anti-angiogensis and the anti-tumor concentration were compared among three extracts.Methods MTT array was used to detect the inhibitory effects of melittin,norcantharidin and bufalin on the growths of human hepatic cancer ECV304 cells in different concentrations and times.The IC50 was then calculated,and the relations of dose-effect and time-effect were observed.Results Three extracts have different degrees of inhibition on the growth of ECV304 cells（P0.05,P0.01） in concentration and time dependent manners.The IC50 of melittin was 7.64,4.25 and 2.64 μg/ml in ECV304 cell inhabitation at 24,48,72 h,that of norcantharidin 271.52,108.83 and 80.76 μg/ml,and that of bufalin 1.104,0.355 and 0.0905 μg/ml,respectively.Conclusion Melittin,norcantharidin and bufalin have definite effects on inhibiting vascualr endothelial cell proliferation and anti-angiogensis.