固定化多酚氧化酶填充床反应器连续制备茶黄素

Study on Continuous Preparation of Theaflavins by Immobilized Polyphenol Enzyme in Packed Bed Reactor

研究了填充床反应器上固定化漆酶连续氧化茶多酚制备茶黄素,自制了连续化制备茶黄素的填充床生物反应器。考察了树脂固定化漆酶在填充床反应器上连续制备茶黄素的主要影响因素如温度、底物流速、氧气流量、pH。研究结果表明:连续化制备体系是可行的,并且在固定化漆酶柱床体积为100 mL时,温度在28℃,底物流速为2.0 mL/min,氧气流量为55 mL/min,pH5.6,此时儿茶素转化率达20.99%。为茶黄素的制备提供一种可行的连续化方法,同时为开拓中式乃至工业生产茶黄素提供一种思路。

Continuous preparation of theaflavins by immobilized polyphenol enzyme in packed bed bioreactor were performed in this paper and The apparatus of continuous preparation system was designed and maded by ourself. In this study, we used D152（maeroreticular weak acidic acrylic acid cation exchange resin） as a matrix to immobolize laccase extracted from Trametes spp. Then we utilized this bio-materials to transformat tea catechins into theaflavins consecutively in a packed bed reactor. Four mainly facts were eonsiderated in this experiment as following： temperature, substrate flow ratio,oxygen flow rate,pH and subsrate concentration. As a results,we selected the temperature at 28℃ ,substrate flow ratio 2.0 mL/min, oxygen flow rate 55 mL/min,pH 5.7 and substrate concentration 10mg/ mL as a optimized condition to biosynthesize theaflavins at a highly efficient. This experiment conditions will give a reference for large scale producing theaflavins basically.