AQP1在CD-1纯系野生型孕鼠胎盘组织的表达

Expression and Distribution of Aquaporin 1 in Placenta of Pure Wild-Type Pregnant Rats

目的:研究水通道蛋白1(Aquaporin 1,AQP1)在小鼠胎盘组织的分布及表达,初步探讨AQP1在羊水循环及母胎液体平衡中的作用。方法:各取四只雌雄成年健康野生型CD1小鼠(wild type,AQP1+/+)及AQP1基因敲除小鼠(AQP1-KO,AQP1-/)-,将纯合子AQP1基因敲除雌雄小鼠等数量合笼交配,第二日检出阴道栓者记为妊娠第1天(1 gestational day,1GD);野生型小鼠同样合笼记录。分别取两组13GD孕鼠的胎盘组织各一个,应用逆转录-聚合酶链反应(RT-PCR)技术及免疫组织化学技术检测AQP1胎盘组织中的表达,并确定AQP1在小鼠胎盘组织的定位。结果:1.RT-PCR结果表明AQP1在CD-1野生型孕鼠胎盘组织表达,AQP1基因敲除鼠无表达;2.免疫组织化学方法发现AQP1表达于小鼠胎盘血管内皮细胞和滋养细胞,AQP1基因敲除鼠无表达。结论:在mRNA水平和蛋白水平均发现AQP1在CD-1纯系野生型孕鼠胎盘组织的表达,提示AQP1可能在羊水循环及母胎液体平衡中发挥作用。

Objective： To detect expression and distribution of AQP1 in mouse placenta,and try to suggest the role of AQP1 in homeostasis of maternal-fetal fluid exchange.Methods： Homozygous AQP1 knockout mice（n=4） and wild type mice（n=4） were mated respectively.The day a copulation plug was found was designated as gestational day 1（1GD）.Four cases of placenta from 13GD were acquired after surgery respectively.The expression of AQP1 in placenta was examined by reverse polymerase chain reaction（RT-PCR）.The slides of placenta were tested by immunohistochemical staining for the expression of AQP1 in placenta tissue.Results： 1）.AQP1 expressed in placenta tissues of wild type mice at RNA and protein levels,without expression in AQP1-KO mice.2）.AQP l was located at the vascular endothelial cell and trophocyte of placenta,no staining in negative group.Conclusion： In this study AQP1 is indicated to be expression in placenta tissues in RNA and protein level.It is suggested that AQP1 may play an important role in amniotic fluid circula-tion and maternal-fetal fluid balance.