摘要
目的:探讨姜黄素对肝癌HepG2细胞抗癌作用及相关周期蛋白依赖激酶抑制因子P21WAF1/CIP1表达的影响。方法:体外培养肝癌HepG2细胞,用MTT法检测姜黄素对HepG2细胞的抑制作用,以RT-PCR方法检测HepG2细胞中P21WAF1/CIP1mRNA的表达,用免疫细胞化学检测其P21WAF1/CIP1蛋白的表达。结果:姜黄素呈时间剂量性抑制HepG2细胞的生长,并显著上调HepG2细胞中P21WAF1/CIP1mRNA和蛋白的表达。结论:姜黄素能抑制HepG2细胞的生长,并上调其中P21WAF1/CIP1的表达。
Objective： To study the anticancer activities of curcumin on human liver carcinoma cell line HepG2 and the effects of express of P21WAF1/CIP1.Methods： Human liver carcinoma HepG2 cell line was cultured in vitro,proliferation inhibitory effect was tested by MTT assay.The express of P21WAF1/CIP1 mRNA was determined by RT-PCR and the express of P21WAF1/CIP1 protein was messured by im-munohistochemistry.Result： The proliferation of HepG2 cells was inhibited by curcumin with dose and time-related effects,Curcumin significantly improve the express of P21WAF1/CIP1 mRNA and protein.Conclusion： Curcumin can inhibit The proliferation of Human liver carcinoma HepG2 cell line and improve the expressION of P21WAF1/CIP1 mRNA and protein.
出处
《现代生物医学进展》
CAS
2011年第14期2658-2660,共3页
Progress in Modern Biomedicine
