Ⅶ因子活化蛋白酶对肺纤维母细胞功能的影响及其临床意义

The effect and clinical significance of factor VII activating protease to lung fibroblast

目的 探讨Ⅶ因子活化蛋白酶（FSAP）对肺纤维母细胞增殖和迁移的影响及其在人类急性肺损伤和肺纤维化过程中的可能作用.方法 体外培养人肺纤维母细胞,分别用CCK8法和Transwell小室法测定FSAP对人肺纤维母细胞增殖和迁移的影响;PCR法检测FSAP对PDGF-BB介导的人肺纤维母细胞胶原Ⅲ mRNA表达的影响.结果 FSAP能抑制人肺纤维母细胞的增殖、迁移.并能抑制PDGF-BB引起的人肺纤维母细胞胶原Ⅲ合成.结论 FSAP能抑制人肺纤维母细胞活化,提示FSAP可能与肺损伤时肺纤维化形成有关.

Objective to explore the effects of factor VII- activating protease （FSAP） on the proliferation and migration of human Lung fibroblast and its possible functions in human acute lung injury and pulmonary fibrosis process. Methods detect the changes of human Lung fibroblast with or without FSAP use CCK8 and Transwell room methods in vitro; use PCR method to detect the PDGF - BB mediated human lung fibroblast collagen III mRNA expression. Results FSAP can restrain human lung fibroblast proliferation and migration and inhibiting lung 6- broblast collagen III synthesis caused by PDGF- BB. Conclusion FSAP can restrain human lung fibroblast activation, which indicate FSAP may involved in the process of pulmonary fibrosis in human lung injury.