类风湿关节炎患者外周血Th17细胞的检测及其临床意义

The clinical significance of increased expression of peripheral blood Th17 cells in patients with rheumatoid arthritis

目的探讨Th17细胞与类风湿关节炎(RA)疾病的相关性。方法采用RT-PCR检测外周血单个核细胞(PBMCs)中Th17细胞的特异性转录因子视磺醛酸相关的孤儿核受体(RORγt)mRNA;酶联免疫吸附法(ELISA)检测20例健康体检者和22例RA患者外周血单个核细胞(PBMCs)刺激培养后,上清液中白细胞介素-17(IL-17)的水平。结果与健康对照组相比,RA患者PBMCs刺激后产生IL-17的量明显增高(P<0.01),RORγtmRNA也高于健康对照组(P<0.01)。结论 Th17细胞参与了RA发病及病情发展。

Objective To study the relationship between increased expression of peripheral blood Th17 cells and the activity of rheumatoid arthritis（RA）.Methods Concentration of IL-17 in the PBMCs culture supernatant（stimulated with anti-CD3 and anti-CD28 mAb） and expression of RORγt mRNA were determined by ELISA and RT-PCR respectively in 22 patients with RA and 20 health controls.Results The expressions of RORγt mRNA and concentration of IL-17 in the patients with RA stimulated by PBMCs were significantly higher than those of controls（P0.01）.Conclusion Th17 cells might play an important role in the activation of the disease in patients with RA.