调节性T细胞及其细胞因子在慢性无菌性前列腺炎的表达及意义

Expression and significance of regulatory T cells and it's cytokines in the chronic abacterial prostatitis

目的观察调节性T细胞(Tr)在慢性无菌性前列腺炎(CAP)发病机制的作用及意义。方法使用流式细胞术检测20例CAP患者及10例健康志愿者外周血Tr的数量;用RT鄄PCR法检测Tr的调节蛋白Foxp3mRNA的表达含量;用ELISA法两组前列腺按摩液细胞因子白介素鄄10(IL鄄10)及转化生长因子鄄β1(TGF鄄β1)的含量;同时比较两组血常规、C鄄反应蛋白(CRP)及血沉(ESR)的比较。结果与健康志愿者比较,CAP患者外周血Tr数量及Foxp3mRNA表达含量明显降低(P=0.013,0.000);细胞因子方面,CAP患者外周血及前列腺液IL鄄10及TGF鄄β1均显著低于志愿者;两组的白细胞(WBC)、中性粒细胞(NEU)、淋巴细胞(LYM)、CRP及ESR均无组间差异;但CAP组患者单核细胞(MONO)明显高于志愿者;相关性分析表明患者单核细胞与Tr、Foxp3、IL-10及TGF-β1呈显著正相关性。结论 Tr数量降低所致的免疫抑制不足,其细胞因子IL-10减少所致MONO数量增加、抗原提呈过度导致免疫反应过强,可能是CAP的重要免疫学机制。

Objective To explore the effect of pathogenesis and significance of regulatory T cells（Tregs） on chronic abacterial prostatitis patients（CAP）.Methods Twenty CAP patients and ten health volunteers were taken into this study and their peripheral blood was collected for testing the Tregs through flow cytometry.Foxp3,the important regulatory factor of Tregs was detected by RT-PCR.The Tregs cytokines,including IL-10 and TGF-β1 in both peripheral blood（PB） and prostatic fluid（PF） were measured by ELISA.Routine blood test（BT）,C-reaction protein（CRP） and erythrocyte sedimentation rate（ESR）were also compared as the inflammatory indexes.Results Compared with the volunteers,quantity of Tregs in the CAP patients were significantly lower than that health volunteers in the Foxp3（P=0.013 and 0.000 respectively）.About the cytokines,the IL-10 and TGF-β1 in both PB and PF of the CAP group were significantly lower than that in the volunteers.There was no statistically difference in the WBC,NEU,LYM,CRP and ESR between the two groups.But in the monocyte,the CAP group was significantly higher than that in the volunteers.Pearson correlation analysis showed that monocyte correlated with the Tregs,Foxp3,IL-10 and TGF-β1 display a positive correlation.Conclusion Because of decreasing Tregs＇ quantity,it causes insufficiency of immunodepression and diminution of Tregs IL-10 would lead to increase of monotype and antigen presents excessive immuno-reaction.It may be an important immunopathogenesis of CAP.