摘要
目的探讨癌基因C-erbB-2及抑癌基因p16在结肠癌组织中表达的相关性,初步分析二者与结肠癌临床分期及预后的关系。方法采用免疫组化ElivisionTM plus法检测50例原发性结肠癌及50例同期结肠癌旁组织中C-erbB-2及p16基因的表达。结果 1.癌基因C-erbB-2在结肠癌组织中的阳性表达率高于癌旁组织,而抑癌基因p16在结肠癌组织中的阳性表达率低于癌旁组织,二者在结肠癌和癌旁组织中的表达有显著统计学差异(P<0.01);2.C-erbB-2在中低分化腺癌、伴有淋巴结转移、肿瘤浸润至浆膜层以及DukesC+D期中表达升高(P<0.05);而p16在高分化腺癌、无淋巴结转移、肿瘤浸润未达浆膜层以及DukesA+B期中表达升高(P<0.05);二者的阳性表达均与患者性别、年龄及肿瘤大小无关(P>0.05);3.C-erbB-2和p16在结肠癌中的表达呈显著负相关(P<0.01)。结论 1.C-erbB-2和p16均与结肠癌发生、发展、浸润和转移密切相关,C-erbB-2表达上调和p16表达下调是结肠癌细胞重要的恶性生物学特征;2.联合检测C-erbB-2和p16表达情况可以作为判断肿瘤恶性程度、侵袭及预后的重要指标;3.C-erbB-2基因的高表达可能是导致p16基因失活的重要因素,通过阻断C-erbB-2基因的致癌途径,有可能提高p16抑癌能力,从而抑制肿瘤的发展;4.C-erbB-2基因有可能作为结肠癌分子靶向治疗的重要指征。
Objective To explore the correlation between the cancer gene C-erbB-2 and the anti-Cancer gene p16 in colon cancer,and analysis their association with clinical stage and prognosis.Methods The Immunohistochemical ElivisionTM plus method has been adopted to analyze fifty patients.Both genes in primary colon tumor and adjacent tissues around those tumors were analyzed.Results The cancer gene C-erbB-2 expressed more in the tumor than it in adjacent tissues,while the anti-cancer gene p16 expressed less in the tumor than it in adjacent tissues.The manifestation rates of the two genes in the primary tumor and adjacent tissues were both significant difference(P0.01).C-erbB-2 genes expressed more in medium and low differentiation colon caner,colon cancer with lymph node metathesis,colon cancer with infiltration to serosa,and Ducks C+D stage cancer patients(P0.05),while p16 genes expressed more in high differentiation colon cancer,colon cancer without lymph node metathesis,colon cancer without infiltration to serosa,and DucksA+B stage patients.Both not related with gender,age and tumor size of patients(P0.05).The two genes are negatively correlated(P0.01).Conclusion The implication is that the higher appearance rates of C-erbB-2 or the lower appearance rates of p16 are malicious biological feature of colon cancer.Results of the joint test of the two genes can serve as indexes for the assessment of the maliciousness of the tumor,infiltration and prognosis.A higher appearance of C-erbB-2 gene may contribute to the inactivation of p16 gene.Therefore,cutting off the cancerogenic channel of C-erbB-2 gene can possibly promote the anti-cancer gene p16,thereby holding up the growth of tumor.C-erbB-2 gene can serve as an important index of molecule targeted therapy of colon cancer.
出处
《青海医学院学报》
CAS
2011年第2期93-97,共5页
Journal of Qinghai Medical College