摘要
选取微波功率、提取温度、提取时间和料液比作为因素,以毛细管电泳-紫外检测测定龙须藤多糖水解产物中的单糖峰面积总和为指标,通过单因素和正交试验,对提取条件进行了优化.结果表明,最大影响因素为微波功率,其次为提取温度,再次为提取时间,最后为料液比.最优提取条件为微波功率600 W,提取温度70℃,提取时间25 m in,料液比1:35.
In order to optimize the microwave extraction conditions of polysaccharides from Bauhinia championii Benth,different factors affecting the extraction efficiency,such as the extraction time,solid-liquid ratio,microwave power,extraction temperature,were selected as the levels of single factor.The single factor and orthogonal tests were used to investigate the optimal microwave extraction conditions indicated by the total peak areas of monosaccharide in the hydrolyzed saccharides from Bauhinia championii Benth polysaccharide using CE equipped with a UV detector.The experiment results showed that the microwave power has the greatest impact on extraction efficiency,followed by the extraction temperature,extraction time and solid-liquid ratio.Therefore,the optimal macrowave power,extraction temperature,extraction time and solid-liquid ratio were 600 W,70 ℃,25 min,1∶35,respectively.
出处
《分析测试技术与仪器》
CAS
2011年第2期92-96,共5页
Analysis and Testing Technology and Instruments
基金
福建省新世纪优秀人才支持计划项目(XSJRC2007-13)
福州大学人才基金(XRC-O711)
陈可冀中西医结合发展基金资助项目(CKJ2007020ZXYJH200710)
关键词
微波提取
龙须藤
多糖
毛细管电泳
microwave extraction
Bauhinia championii Benth
polysaccharides
capillary electrophoresis
