Survivin、Bax蛋白在喉病变组织中的表达及临床意义

Expression of Survivin and Bax protein in differ laryngeal disease and its clinical significance

目的检测声带息肉、喉不典型增生及声门区喉癌中凋亡抑制蛋白(Survivin)和凋亡促进蛋白(Bax)的表达,探讨它们在疾病发生、发展中的作用及之间的相关性和临床意义。方法采用免疫组化SP法对声带息肉、喉不典型增生、声门区喉癌各24例进行Bax与Survivin的检测及分析。结果声门区喉癌组中Survivin蛋白的阳性表达率为70.83%,明显高于声带息肉组(25.00%)与喉不典型增生组(58.33%)。Survivin蛋白阳性组和阴性组病例中,临床分期在两组间差异有统计学意义(P=0.011 1),而年龄分布与性别因素在两者间差异无统计学意义(P>0.05)。Bax蛋白在声带息肉组中阳性表达率为87.5%,明显高于声门区喉癌组(41.67%)与喉不典型增生组(54.17%)。Bax阳性与阴性患者相比,临床分期在两组间差异有统计学意义(P=0.018 3),而性别分布与年龄因素在两者间差异无统计学意义(P>0.05)。结论 Survivin和Bax同时参与了喉癌细胞凋亡的调控,二者呈负性相关,并对喉癌的发生发展起着重要作用。

Objective To examine the expression of apoptosis inhibitive protein（survivin）and pro-apoptosis protein（bax）in polyp vocal cord and displasia and glottic carcinoma,and investigate the relationship between the expression level of them and the clinical significance.Methods Immunohistochemical SP method was used to determine the expressions of Bax and Survivin proteins in polyp vocal cord and displasia and glottic carcinoma of 72 cases.Results Survivin protein was expressed in 24 samples of glottic carcinoma（70.83%）,which remark higher than the polyp vocal cord（25.00%）and displasia（58.33%）.There were no differences of gender,age of glottic carcinoma between negative and positive of survivin protein groups（P0.05）,but we found that there were different effect of clinical stage between the two groups（P=0.0111）.Bax protein was expressed in 24 samples of polyp vocal cord（87.5%）,which remark higher than the glottic carcinoma（41.67%）and displasia（54.17%）.There were no differences of gender,age of glottic carcinoma between negative and positive of bax protein groups（P0.05）;but we found that there were different effect of clinical stage between the two groups（P=0.0183）.Conclusion The both of survivin and bax were involve in regulation of the apoptosis and cell cycle of carcinoma of the larynx,which indicate that it may play a crucial role in the genesis and progression of 1aryngeal neoplasms.