杜仲总黄酮对成骨细胞增殖及Ⅰ型胶原蛋白表达的影响

Effects of total flavonoids of Eucommiabark on the proliferation of rat osteoblast and synthesis of Ⅰ collagen protein

目的了解杜仲总黄酮对新生大鼠颅骨体外培养成骨细胞的增殖及合成胶原蛋白的影响。方法杜仲总黄酮以质量浓度10,100,200和400μg·mL-1分别加入从2 d的SD大鼠颅骨分离培养的成骨细胞中,用MTT法评价对成骨细胞增殖的影响;采用激光共聚焦扫描显微镜检测Ⅰ型骨胶原蛋白的表达情况。结果与对照组比较,质量浓度10200μg·mL-1的杜仲总黄酮能明显促进成骨细胞的增殖（P〈0.05）,但并不能显著地促进成骨细胞合成Ⅰ型胶原蛋白。

Objective To investigate the effects of total flavonoids of Eucommia bark on proliferation of rat osteoblast and synthesis of collagen protein.Methods The osteoblast was isolated from the crinial bone of Sprague-Dawley rats at postnatal day 2,identified by alkaline phosphatase staining and cultured with 10,100,200 and 400 μg·mL-1 total flavonoids of Eucommia bark.The proliferation of cells was identificated by ELISA using MTT,and the synthesis of Ⅰ collagen protein was detected by confocal laser scanning microscopy.Results The total flavonoids of Eucommia bark at the concentration from 10 μg·mL-1 to 200 μg·mL-1 improved evidently the growth and proliferation of rat osteoblast but didn’t have obvious promoting effect to the synthesis of Ⅰ collagen protein.Conclusion The flavonoids of Eucommia bark are useful for the supporting of bone through promoting the quantity of osteoblasts