局灶脑缺血再灌注后成年大鼠大脑MyT1表达变化

Brain expression of MyT1 in adult rats after focal cerebral ischemia and reperfusion

目的探讨脑缺血再灌注后成年大鼠大脑MyT1表达变化及其意义。方法以线栓法制作成年SD大鼠局灶性脑缺血再灌注模型（阻塞90rain再灌注1，7，14d）；用免疫荧光组织化学法检测脑缺血再灌注成年大鼠早期大脑梗死中心区、梗死周边区和缺血对侧区MyT1阳性细胞数量。结果（1）脑缺血再灌注后各时间点梗死中心区MyT1阳性细胞数明显减少；（2）脑缺血再灌注后梗死周边区MyT1阳性细胞数从脑缺血后第1天开始增加；脑缺血后7d和14d时，各组大鼠MyT1阳性细胞增加数较之对照组大鼠有显著的差别。结论成年SD大鼠脑缺血再灌注后梗死周边区MyT1表达显著增加，可能参与了少突胶质前体细胞的发育过程，调节髓鞘蛋白基因的转录，参与缺血性脑损伤的修复过程。

Objective To explore the early phase expression and the significance of MyT1 in adult rats after focal cerebral isehemia and reperfusion. Methods Focal cerebral ischemia and reperfusion mod- el in male adult SD rats was induced by occluding the right middle artery with 0/4 nylon thread （blood flow blocked for 90 minutes and then reperfusion for 1 day,7 day and 14 day）. MyT1 --positive cells in the ischemic core, ischemic penumbra and contralateral area was examined at each time--point after reperfusion by immunofluorescence histochemistry. Results The number of MyT1 --positive cells within the ischemic core decreased significantly at each time--point after reperfusion comparing with the control group. The number of MyT1 mRNA --positive cells within ischemic penumbra increased at 1 day reper- fusion and increased significantly at the day of 7 and 14 after reperfusion comparing with the control group. Conclusions The early phase expression of MyT1 increases in the peri--infarct area in the adult rat brain and maybe involve in the developing of oligodendrocyte precursor cells, which may regulate the transcription of myelin protein genes and contribute to the post--ischemic repairing of the brain injury.