贴敷式头部亚低温不同时间窗对新生仔兔HIBD治疗时NSE变化的研究

NSE study in different time windows of cephalic--applied sub--hypothermia in HIBD therapy in newborn rabbits

目的通过贴敷式头部亚低温不同时间窗对新生仔兔HIBD（缺血缺氧性脑病）治疗前后血中NSE（神经元特异性烯醇化酶）的测定，揭示贴敷式头部亚低温对新生仔兔HIBD有保护作用。方法采用酶联免疫法测定不同时间窗时间的血清中NSE。贴敷式头部亚低温实施6h治疗。结果HIBD后不同时间窗组及假手术组（正常组）组间比较，血清中NSE含量有显著差异（P〈0．01），经q检验进行两两比较发现，HIBD后3h组NSE含量与正常组未见显著差异（P〉0．05），而HIBD后其他组与正常组间均有显著性差异。结论贴敷式头部亚低温对缺血缺氧性脑病有保护作用；HIBD后不同时间窗均有脑保护作用，但HIBD后3h组脑保护作用最强。

Objective To explore the protective effect of sticking--type head cooling, by measuring the content of the NSE in different time windows of cephalic--applied sub--hypothermia in the HIBD therapy in newborn rabbits. Methods a cephalic-- applied sub-- hypothermia was administered to new- born rabbits for 6 hours, while gauging the NSE concentration in serum in different time windows by ELISA （enzyme linked immunosorbent assay）. Results There is a significant difference in NSE concentration between false--operational group（the normal group） and diverse time--window groups （P〈 0.01 ）. No significant difference was detected in the NSE concentration between the normal group and the three--hour--after--HIBD group by Q Test （P〉0.05）, whereas other post--HIBD groups did have significant differences from the normal group. Conclusions Cephalic--applied sub--hypothermia contrib- uted to the protection of HIBD, that carries out its function in different time windows,among those the three--hours being the most powerful one.