nm23-H1蛋白在宫颈上皮内瘤变及宫颈癌中的表达及临床意义

The expression and clinical significance of nm23-H1 protein in cervical intraepithelial neoplasia and cervical cancer

目的探讨宫颈上皮内瘤变及宫颈癌中肿瘤转移抑制基因nm23-H1蛋白(nonmetastatic protein 23 homologue 1)的表达,试图阐明nm23-H1蛋白的表达在宫颈癌发生、发展及预后的作用。方法采用免疫组化SP法检测宫颈鳞状上皮癌60例、宫颈上皮内瘤变60例(CINⅠ、CINⅡ、CINⅢ各20例)、正常宫颈上皮组织20例中nm23-H1蛋白的表达,并分析nm23-H1蛋白的表达与临床病理特征的关系。结果 nm23-H1蛋白在宫颈癌中的表达明显低于正常宫颈组织和宫颈上皮内瘤变CINⅠ及CINⅡ(P<0.05),CINⅢ中nm23-H1蛋白阳性表达率明显减少,但与宫颈鳞状上皮癌相比,差异无统计学意义(P>0.05)。宫颈鳞状上皮癌中nm23-H1的表达与淋巴结转移及FIGO分期有关(P<0.05),与患者年龄、肿瘤的组织病理分级及肿瘤大小无关(P>0.05)。结论 nm23-H1蛋白的低表达是宫颈癌的一个高危因素,对判断宫颈癌淋巴结转移和估计预后是一项有用的指标。

Objective To investigate the roles of anti-metastasis gene-nm23-H1 protein（non-metastatic protein 23 homologue 1） in the tumorigenesis,progress and prognosis of cervical cancer by observing its expression in cervical intraepithelial neoplasia and cervical squamous cell carcinoma.Methods The expression of nm23-H1 protein was detected by immunohistochemistry（SP） method in 60 cases of squamous cell carcinomas,60 cases of cervical intraepithelial neoplasia（20 CINⅠ,20 CINⅡand 20 CINⅢ,respectively） and 20 cases of normal cervical tissues,and the clinicopathological significance of the nm23-H1 protein expression was analyzed.Results The positive expression rate of nm23-H1 protein in cervical cancer tissues was significantly lower than that of normal cervical tissue,especially in CINⅠand CINⅡ（P0.05）.The positive expression rate of nm23-H1 protein in CINⅢ was lower than that in cervical cancer tissues,however,there was no significant difference between them（P0.05）.The expression of nm23-H1 in cervical cancer tissues was closely correlated with lymph node metastases and FIGO stages（P0.05）,however,which was not related with patients＇ age,histopathological grades and tumor size（P0.05）.Conclusion The low expression of nm23-H1 protein is a high risk factor of cervical cancer,which is a significant index for evaluating lymph node metastases and patients＇ prognosis in patients with cervical cancer.