黄瓜新品种宁运3号的组织培养和快速繁殖

Study on Tissue Culture and Rapid Propagation of Cucumber Variety Ningyun No.3

以黄瓜新品种宁运3号的种子为材料,进行组织培养和快速繁殖研究,比较了不同时间HgCl2溶液浸泡对种子萌发的影响,不同浓度的6-BA、NAA对不定芽及根诱导的影响。结果表明,用0.1 g/100mLHgCl2浸泡8 min,种子的萌发率较高,污染率较低;在0.1～1.0mg/L范围内随6-BA浓度的提高,不定芽诱导率提高,MS+6-BA 1.0 mg/L为较适宜的芽诱导培养基;1/2MS+NAA 0.5 mg/L对生根诱导效果较好。

Using the seed of the new cucumber variety Ningyun No.3 as material,tissue culture and rapid reproduction was studied.The effect of different immersing time in HgCl2 on seeds germination,influence of different concentrations of 6-BA,NAA on bud and root induction was studied.The results showed that immersed in 0.1 g/100mL HgCl2 for 8 min,relatively high germination ratio with low pollution rate could be achieved;In 0.1~1.0 mg/L,the induction rate of adventitious buds increased by the increasing of 6-BA＇s concentration;MS＋1.0 mg/L 6-BA was the best medium for crowd bud propagation.The optimized medium for rooting was 1/2 MS＋0.5 mg/L NAA.