摘要
从川芎中分离制备洋川芎内酯H和洋川芎内酯I对照品.用8倍量80%乙醇回流提取川芎药材,得到川芎浸膏经D-101大孔吸附树脂、硅胶柱色谱及反相高效制备液相色谱分离,获得两个化合物单体.经核磁共振波谱分析,确定其分别为洋川芎内酯H和洋川芎内酯I,两者纯度均高于98%,可作为对照品进行定量分析.该方法操作简单,重现性好,上样量大,能够同时获得大量的高纯度的目标成分,为洋川芎内酯H和洋川芎内酯I单体的制备研究提供了一种新的方法.
Senkyunolide H and I were separated and purified from Ligusticum chuanxiong by using 8 times the amount of 80% ethanol to extract Ligusticum chuanxiong Hort.The ethanol extract was subjected to D101 macroporous adsorption resin,silica gel,and preparative reversed-phase HPLC for the isolation of target compounds.Their structures were elucidated as senkyunolide H and I by NMR spectral analysis.And the purity of each compound was above 98%.This method is simple,effective,and reliable for the preparation of senkyunolide H and I simultaneously with good reproducibility and high purity.
出处
《浙江工业大学学报》
CAS
北大核心
2011年第4期386-389,共4页
Journal of Zhejiang University of Technology
基金
国家重大科技专项"重大新药创制"资助项目(2009ZX09313-036)
关键词
反相高效制备液相色谱法
川芎
洋川芎内酯H
洋川芎内酯I
preparative reversed-phase high performance liquid chromatography
Ligusticum chuanxiong Hort
senkyunolide H
senkyunolide I
